5/6/2023 0 Comments Kid3 beige adipocyte![]() Among the various populations, we identify a subset of cells expressing the mature adipocyte marker alanine serine cysteine transporter-1 ( Asc-1), which we show to be enriched in the adolescent SVF and to favor white over beige adipocyte differentiation. Here, we show that single-cell transcriptome profiling by single-cell RNA sequencing (scRNA-seq) of adolescent and adult stromal vascular fraction (SVF) from subcutaneous white adipose tissue of C57Bl/6 mice reveals major differences in adipocyte precursor populations. However, these dramatic changes in cellular composition and size of adipose tissues, especially subcutaneous adipose tissue, have not been studied in detail to elucidate novel mechanisms enabling healthy adipose tissue expansion in adults. Moreover, C57Bl/6 mice, among other strains, show a transient peak in the number of thermogenic beige adipocytes ~14 days postpartum, which decreases thereafter, when mice are housed at room temperature 12. However, adipose tissues show a dramatic, but healthy expansion in the first postnatal weeks, which is essential for the establishment of a functional metabolism. Previous studies mainly focused on differences in precursor and adipocyte populations in adult mice in the transition from normal weight to obesity and insulin resistance. Thus, the type of de novo differentiating adipocytes could strongly affect the metabolic response of the organism to weight gain, as lipid storage capacity, as well as adipokine production differs between white adipocyte subtypes 6, 7, 8, 9. However, recent data indicate the existence of multiple white 6, 7, 8, 9 and brown 10, 11 adipocyte subtypes that originate from distinct precursor populations. A key element of healthy adipose tissue expansion is de novo differentiation of adipocytes rather than storage of fat in already existing adipocytes 4, 5. Increasing evidence in humans and rodents suggests that maintaining adipose tissue function dissociates body fat mass gain from the development of metabolic complications enabling a “fat-fit” phenotype 1, 2, 3. Mechanistically, this was mediated by a function of the amino acid transporter ASC-1 specifically in proliferating preadipocytes involving the intracellular accumulation of the ASC-1 cargo D-serine. Loss of Asc-1 in subcutaneous preadipocytes resulted in spontaneous differentiation of beige adipocytes in vitro and in vivo. We identified a subset of adolescent preadipocytes expressing the mature white adipocyte marker Asc-1 that showed a low ability to differentiate into beige adipocytes compared to Asc-1 negative cells in vitro. To understand the differences between adolescent and adult adipose tissue expansion, we studied the cellular composition of the stromal vascular fraction of subcutaneous adipose tissue of two and eight weeks old mice using single cell RNA sequencing. However, white adipose tissue expansion at early ages is essential to establish a functional metabolism. Nature Communications volume 12, Article number: 1588 ( 2021)Īdipose tissue expansion, as seen in obesity, is often metabolically detrimental causing insulin resistance and the metabolic syndrome. Ahmed Elagamy Mohamed Mahmoud Khalil 1, 2,.Asc-1 regulates white versus beige adipocyte fate in a subcutaneous stromal cell population ![]()
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